Journal: Journal of Immigrant and Minority Health

Article Title: Latent Tuberculosis Infection in a Migrant Agricultural Community in Baja California, Mexico

doi: 10.1007/s10903-010-9372-2

Figure Lengend Snippet: Characteristics by QuantiFERON ® -TB Gold In-Tube (QFT) status among residents of an agricultural community in San Quintín, Mexico: October, 2008

Article Snippet: M. tuberculosis infection was detected by the QuantiFERON ® -TB Gold In-Tube interferon gamma release assay (Cellestis, Ltd. Carnegie, Australia), an in vitro system that detects interferon gamma released by whole blood samples after introduction of ESAT-6, CFP-10, and TB7.7 proteins that mimic antigens specific to the M. tuberculosis complex present in both TB and LTBI [ ].

Techniques: Medications

Journal: Journal of Immigrant and Minority Health

Article Title: Latent Tuberculosis Infection in a Migrant Agricultural Community in Baja California, Mexico

doi: 10.1007/s10903-010-9372-2

Figure Lengend Snippet: Multivariate analysis of tuberculosis infection correlates based on QuantiFERON ® -TB Gold In-Tube assay testing among residents of an agricultural community in San Quintín, Baja California, Mexico: October, 2008

Article Snippet: M. tuberculosis infection was detected by the QuantiFERON ® -TB Gold In-Tube interferon gamma release assay (Cellestis, Ltd. Carnegie, Australia), an in vitro system that detects interferon gamma released by whole blood samples after introduction of ESAT-6, CFP-10, and TB7.7 proteins that mimic antigens specific to the M. tuberculosis complex present in both TB and LTBI [ ].

Techniques: Infection

Journal: PLoS ONE

Article Title: A Simple Method to Quantitate IP-10 in Dried Blood and Plasma Spots

doi: 10.1371/journal.pone.0039228

Figure Lengend Snippet: QFT-CMV test stimulated blood from 8 healthy individuals, 5 CMV responders, were used to compare IP-10 signals in DBS and DPS samples. Nil antigen and mitogen samples pooled (n = 24). . Correlation analysis, (p<0.0001, Spearman); Bland-Altman plot of the same data. Each sample is represented on the graph as the average of the two measurements against the difference. The limits of agreement are specified as the average difference (bias, −0.8 pg/ml) ±1.96 standard deviation of the difference, (71 pg/ml).

Article Snippet: QFT-TB and QFT-CMV results in plasma samples were analyzed according to manufacturers guidelines using manufacturers software (v2.61, www.cellestis.com ).

Techniques: Standard Deviation

Journal: PLoS ONE

Article Title: A Simple Method to Quantitate IP-10 in Dried Blood and Plasma Spots

doi: 10.1371/journal.pone.0039228

Figure Lengend Snippet: QFT-CMV responses from 26 healthy individuals (Control group 2) were divided into CMV-positive (n = 17) and CMV-negative (n = 9) based on the QFT-CMV cut off for IFN-γ suggested by the manufacturer (0.2 IU/ml).

Article Snippet: QFT-TB and QFT-CMV results in plasma samples were analyzed according to manufacturers guidelines using manufacturers software (v2.61, www.cellestis.com ).

Techniques:

Journal: The Pediatric infectious disease journal

Article Title: Evaluation of QuantiFERON-TB Gold In-Tube and Tuberculin Skin Tests Among Immigrant Children Being Screened for Latent Tuberculosis Infection

doi: 10.1097/INF.0000000000000494

Figure Lengend Snippet: QFT and TST results for all children within the study population.

Article Snippet: The QFT was processed on-site according to manufacturer’s instructions (Cellestis LTD, Carnegie, Australia).

Techniques:

Journal: The Pediatric infectious disease journal

Article Title: Evaluation of QuantiFERON-TB Gold In-Tube and Tuberculin Skin Tests Among Immigrant Children Being Screened for Latent Tuberculosis Infection

doi: 10.1097/INF.0000000000000494

Figure Lengend Snippet: Percentage of TST+ or QFT+ and Concordance between TST and QFT Results *

Article Snippet: The QFT was processed on-site according to manufacturer’s instructions (Cellestis LTD, Carnegie, Australia).

Techniques:

Journal: The Pediatric infectious disease journal

Article Title: Evaluation of QuantiFERON-TB Gold In-Tube and Tuberculin Skin Tests Among Immigrant Children Being Screened for Latent Tuberculosis Infection

doi: 10.1097/INF.0000000000000494

Figure Lengend Snippet: Proportion of children testing positive, by age and country. The proportion who tested positive by age is denoted with a black dot. Dots are connected with a dashed line for TST and a solid line for QFT. Smoother lines (shaded CIs) are shown for each set of points.

Article Snippet: The QFT was processed on-site according to manufacturer’s instructions (Cellestis LTD, Carnegie, Australia).

Techniques:

Journal: Molecules

Article Title: Development of TaqMan Real-Time Fluorescent Quantitative PCR Method for Identification and Quantification of Sinomenium acutum- Originated Herbal Drugs

doi: 10.3390/molecules30183763

Figure Lengend Snippet: Probe and primer design and screen. ( A ) Location of probes and primers on ITS2 sequence designed for detecting QFT (QT: Qingfengteng ( Sinomenium acutum ); MQT: Qingfengteng ( Sinomenium acutum var. cinereum ); BDG: Beidougen; JST: Jishiteng; KJT: Kuanjinteng; QST: Qingsheteng; HBQFT: Huibeiqingfengteng). ( B ) Comparison of the effectiveness of four groups of probes and primers QFT detection (Group a: QFT-1_P; Group b: QFT-2_P; Group c: QFT-3_P; Group d: QFT-4_P; n = 3).

Article Snippet: Species-specific probes and primers for QFT were designed using BioEdit software (v7.7.1.0).

Techniques: Sequencing, Comparison

Journal: Molecules

Article Title: Development of TaqMan Real-Time Fluorescent Quantitative PCR Method for Identification and Quantification of Sinomenium acutum- Originated Herbal Drugs

doi: 10.3390/molecules30183763

Figure Lengend Snippet: Specificity evaluation. ( A ) Molecular weight of qPCR amplification products by Qsep analyzer. ( B ) Amplification curve of target species and non-target species (target species: QFT, positive; non-target species: Adulterants, negative; n = 3).

Article Snippet: Species-specific probes and primers for QFT were designed using BioEdit software (v7.7.1.0).

Techniques: Molecular Weight, Amplification

Journal: Molecules

Article Title: Development of TaqMan Real-Time Fluorescent Quantitative PCR Method for Identification and Quantification of Sinomenium acutum- Originated Herbal Drugs

doi: 10.3390/molecules30183763

Figure Lengend Snippet: Bikepian and laboratory-prepared QFT aqueous decoctions. ( A-1 ) Amplification curve of Bikepian preparations (n = 3). ( A-2 ) Statistical comparison of BiKePian and laboratory-prepared QFT aqueous decoctions (n = 6). ( B-1 ) Amplification curve of laboratory-prepared QFT aqueous decoction in six different decoction time (n = 3). ( B-2 ) Statistical comparison of laboratory-prepared QFT aqueous decoctions with different decocting time (n = 3). ns represents not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Species-specific probes and primers for QFT were designed using BioEdit software (v7.7.1.0).

Techniques: Amplification, Comparison